Discover recommended standard operating procedures for hatchery-level diagnostic sample processing. Learn best practices for specimen collection, isolation, preservation, and contamination control to support reliable molecular diagnostics and accurate PCR testing.
Step-by-step laboratory guide for hatchery technicians covering proper euthanasia, hepatopancreas dissection, 10% bleach sterilization of surgical instruments to eliminate DNA carryover, and tissue isolation within a biosafety cabinet to minimize aerosol contamination and reduce the risk of PCR false positives.
qPCR vs dPCR vs Nested PCR — what is the difference, and which molecular diagnostic approach is right for shrimp aquaculture? We take a clear look at shrimp molecular diagnostics and the science behind three important PCR technologies.